Table of Contents: “…DT40 gene disruptions: a how-to for the design and the construction of targeting vectors -- Immunoglobulin gene conversion or hypermutation: that's the question -- Genome resources for the DT40 community -- Chromosome engineering in DT40 cells and mammalian centromere function -- Function of RECQ family helicase in genome stability -- Genetic analysis of apoptotic execution -- The DT40 system as a tool for analyzing kinetochore assembly -- Analysing the DNA damage and replication checkpoints in DT40 cells -- Using DT40 to study clathrin function -- Genetic analysis of B cell signaling -- DT40 mutants: a model to study transcriptional regulation of B cell development and function -- Transcription and RNA processing factors play complex roles in DT40 cells -- Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions -- Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray -- Calcium signaling, ion channels and more -- Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development -- The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line -- Phenotypic analysis of cellular responses to DNA damage -- ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells -- Stable non-targeted transfection of DT40 -- Basic cell culture conditions -- Excision of floxed-DNA sequences by transient induction of MER-CRE-MER -- Immunoglobulin gene conversion and hypermutation assay by facs -- Target screening by PCR -- Mitotic index determination by flow cytometry -- Centrifugal elutriation as a means of cell cycle phase separation and synchronisation -- Preparation of genomic DNA for microarray-based comparative genome hybridization -- Analysis of cellular Mg2+ in DT40 cells -- Transient transfection of DT40 -- Retroviral transduction of DT40 -- Colony survival assay -- Subcloning DT40 by limiting dilution -- Subnuclear immunofluorescence -- Sister chromatid exchange assay -- 2D cell cycle analysis -- Purification of tap-tagged proteins by two-step pull down from DT40 cells -- Synchronization of cells -- Targeted transfection of DT40 cells -- Luciferase reporter assay -- Indirect immunofluorescence microscopy -- Quantification of receptor-mediated endocytosis -- Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation -- Isolation of nuclear and cytoplasmic proteins from DT40 cell lines.…”
Connect to this title online

Table of Contents: “…-Reductase Inhibitors -- Androgen Receptor Structure/Function Relationships -- Insights from AR Gene Mutations -- Functional Motifs of the Androgen Receptor -- The Role of the Androgen Receptor Polyglutamine Tract in Prostate Cancer: In Mice and Men -- The Androgen Receptor Coactivator-Binding Interface -- Co-Regulators of the Androgen Receptor -- Coregulators and the Regulation of Androgen Receptor Action in Prostate Cancer -- Androgen Receptor Coregulators and Their Role in Prostate Cancer -- Interaction of the Androgen Receptor Ligand-Binding Domain with the N-Terminal Domain and with Coactivators -- Multitasking and Interplay Between the Androgen Receptor Domains -- Chromatin Remodeling and Androgen Receptor-Mediated Transcription -- Ligand-Independent Activation of the Androgen Receptor -- Ligand-Independent Androgen Receptor Activity -- Role of IL-6 in Regulating the Androgen Receptor -- The Role of Cyclic AMP in Regulating the Androgen Receptor -- Role of the Androgen Receptor in Prostate Cancer during Castration -- Cellular and Molecular Signatures of Androgen Ablation of Prostate Cancer -- Tissue Levels of Androgens in Castration-Recurrent Prostate Cancer -- Unique Effects of Wnt Signaling on Prostate Cancer Cells: Modulation of the Androgen Signaling Pathway by Interactions of the Androgen Receptor Gene and Protein with Key Components of the Canonical Wnt Signaling Pathway -- The Role of Foxa Proteins in the Regulation of Androgen Receptor Activity -- Androgen-Regulated Genes during Prostate Cancer Progression -- Androgen Receptor as a Licensing Factor for DNA Replication -- Androgen-Regulated Genes in the Prostate -- Mapping the Androgen Receptor Cistrome -- Differential Regulation of Clusterin Isoforms by the Androgen Receptor -- Androgen Regulation of Prostate Cancer Gene Fusions -- Androgens and the Lipogenic Switch in Prostate Cancer -- The Androgen Receptor as a Drugable Target -- Molecular Biology of Novel Targets Identified Through Study of Castration-Recurrent Prostate Cancer -- Selenium and Androgen Receptor in Prostate Cancer.…”
Connect to this title online

Table of Contents: “…9 1.6 Conclusions 12 Further Reading 12 2 Matter, the Stuff of Life 13 2.1 Matter and Life 13 2.2 We are Made of Ordinary Matter 13 2.3 Matter: Its Nucleus 14 2.3.1 Isotopes 14 2.4 Electrons, Atoms and Ions 15 2.5 Types of Bonding in Matter 15 2.6 Ionic Bonding 15 2.6.1 Ionic Bonds and Life 16 2.7 Covalent Bonding 17 2.7.1 Covalent Bonds and Life 17 2.8 Metallic Bonding 19 2.9 van der Waals Interactions 19 2.9.1 Dipole dipole (Keesom) Forces 19 2.9.2 Dipole induced Dipole (Debye; Pronounced deh-beye) Forces 19 2.9.3 Dispersion Forces 20 2.9.4 van der Waals Interactions and Life 20 2.10 Hydrogen Bonding 20 2.10.1 Hydrogen Bonds and Life 21 2.11 The Equation of State Describes the Relationship between Different Types of Matter 21 2.12 Phase Diagrams 22 2.12.1 Matter and Mars 23 2.12.2 Phase Diagrams and Life 24 2.13 Other States of Matter 25 2.13.1 Plasma 25 2.13.2 Degenerate Matter 25 2.14 The Interaction between Matter and Light 27 2.14.1 The Special Case of the Hydrogen Atom 29 2.14.2 Uses to Astrobiology 29 2.15 Conclusions 30 Further Reading 30 3 Life s Structure: Building the Molecules 33 3.1 Building Life 33 3.2 The Essential Elements: CHNOPS 33 3.3 Carbon is Versatile 34 3.4 The Chains of Life 35 3.5 Proteins 35 3.6 Chirality 37 3.7 Carbohydrates (Sugars) 38 3.8 Lipids 39 3.9 The Nucleic Acids 39 3.9.1 Ribonucleic Acid 40 3.10 The Solvent of Life 43 3.10.1 Water as the Best Solvent 43 3.11 Alternative Chemistries 44 3.11.1 Alternative Core Elements 44 3.11.2 Alternative Solvents 47 3.12 The Structure of Life and Habitability 48 3.13 Conclusion 49 Further Reading 49 4 Life s Structure: Building Cells from Molecules 51 4.1 From Molecules to Cells 51 4.2 Types of Cells 51 4.3 Shapes of Cells 53 4.4 The Structure of Cells 53 4.5 Membranes 55 4.5.1 Gram-negative and Positive Prokaryotic Membranes 56 4.5.2 Archeal Membranes 58 4.6 The Information Storage System of Life 58 4.6.1 Transcription DNA to RNA 59 4.6.2 Translation RNA to Protein 60 4.6.3 A Remarkable Code 62 4.6.4 DNA Replication 62 4.6.5 Plasmids 64 4.6.6 eDNA 65 4.7 Cell Reproduction 65 4.8 The Growth of Life 67 4.9 Moving and Communicating 68 4.9.1 Movement in Prokaryotes 68 4.9.2 Communication in Prokaryotes 68 4.10 Eukaryotic Cells 70 4.10.1 Endosymbiosis 72 4.11 Viruses 72 4.12 Prions 74 4.13 Conclusions 74 Further Reading…”
Connect to this title online (unlimited simultaneous users allowed; 325 uses per year)